How to dilute the standard

Dilution method:
i.e. Human Neurogenic Locus Notch Homolog Protein 1 ELISA (Cat#Cat.NO EA3218Hu)
Dilution method: Take 6 tubes, reconstitute the standard with 150 μl of standard diluent to generate a 192ng/ml standard stock solution. Pipette 150ul of the 192ng/ml stock solution to the second tube and mix up to produce a 96 ng/ml working solution. Pipette 150ul of the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don’t pipette any solution into it from the former tube.