Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Primacu > Mouse Semaphorin-4D, Sema4d Primacu™ ELISA Kit

Mouse Semaphorin-4D, Sema4d Primacu™ ELISA Kit

Mouse Semaphorin-4D, Sema4d Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 10.24 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 10.24 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Semaphorin-4D

Code

BPE329

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

10.24 pg/mL

Detection range

46.88-3000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

UniProt accession

MASS(Da)

95640

GeneID

20354

Synonyms

Semacl2;Semaj

Gene names

Sema4d

Research area

Immunology,Neuroscience

Target protein

Semaphorin 4D

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE329 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3000 pg/ml 2.333 2.287 2.198
2.242
1500 pg/ml 1.259 1.209 1.12
1.159
750 pg/ml 0.649 0.663 0.574
0.677
375 pg/ml 0.389 0.372 0.283
0.355
187.5 pg/ml 0.25 0.235 0.146
0.22
93.75 pg/ml 0.167 0.162 0.073
0.157
46.875 pg/ml 0.129 0.124 0.035
0.119
0 pg/ml 0.085 0.089 0
0.093

Precision

We measured random samples of BPE329 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 141 6.3 4.5
Intra-assay 2 20 542 10.4 1.9
Intra-assay 3 20 1869 44.4 2.4
Inter-assay 1 20 140 9.5 6.8
Inter-assay 2 20 539 19.4 3.6
Inter-assay 3 20 1850 75.4 4.1

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE329

    $2320.00

  • 96T

    BPE329

    $580.00

  • 15*96T

    BPE329

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up