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Home > Product > ELISA Primacu > Human Regenerating Islet-Derived Protein 4, REG4 Primacu™ ELISA Kit

Human Regenerating Islet-Derived Protein 4, REG4 Primacu™ ELISA Kit

Human Regenerating Islet-Derived Protein 4, REG4 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 0.98 pg/mL

Detection range: 3.91-250 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 0.98 pg/mL

Detection range: 3.91-250 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Regenerating islet-derived protein 4

Code

BPE183

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

0.98 pg/mL

Detection range

3.91-250 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.

UniProt accession

MASS(Da)

18230

GeneID

83998

Synonyms

GISP;RELP

Gene names

REG4

Research area

Cancer,Immunology

Target protein

REG4

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE183 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
250 pg/ml 3.522 3.369 3.237
3.215
125 pg/ml 2.008 1.937 1.805
1.866
62.5 pg/ml 1.054 1.019 0.887
0.983
31.25 pg/ml 0.64 0.669 0.537
0.697
15.625 pg/ml 0.359 0.352 0.22
0.344
7.8125 pg/ml 0.253 0.238 0.106
0.223
3.90625 pg/ml 0.177 0.185 0.053
0.192
0 pg/ml 0.134 0.132 0
0.129

Precision

We measured random samples of BPE183 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 21.3 1.5 7
Intra-assay 2 20 92.8 4.1 4.4
Intra-assay 3 20 193 7.6 3.9
Inter-assay 1 20 21.1 1.9 9
Inter-assay 2 20 91.8 5 5.4
Inter-assay 3 20 194 13.6 7

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE183

    $2320.00

  • 96T

    BPE183

    $580.00

  • 15*96T

    BPE183

    $6525.00

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Lead time: Within two weeks

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