Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 25000 pg/ml | 2.662 | 2.744 | 2.635 |
| 2.825 | |||
| 12500 pg/ml | 1.606 | 1.604 | 1.495 |
| 1.602 | |||
| 6250 pg/ml | 0.836 | 0.871 | 0.762 |
| 0.905 | |||
| 3125 pg/ml | 0.533 | 0.534 | 0.425 |
| 0.534 | |||
| 1562.5 pg/ml | 0.271 | 0.287 | 0.178 |
| 0.302 | |||
| 781.25 pg/ml | 0.2 | 0.188 | 0.079 |
| 0.175 | |||
| 390.625 pg/ml | 0.148 | 0.155 | 0.046 |
| 0.161 | |||
| 0 pg/ml | 0.112 | 0.109 | 0 |
| 0.105 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 1551 | 135 | 8.7 |
| Intra-assay | 2 | 20 | 3299 | 250 | 7.6 |
| Intra-assay | 3 | 20 | 13748 | 1124 | 8.2 |
| Inter-assay | 1 | 20 | 1533 | 111 | 7.3 |
| Inter-assay | 2 | 20 | 3322 | 261 | 7.9 |
| Inter-assay | 3 | 20 | 13677 | 924 | 6.8 |
Protocols
References
Customer reviews and Q&As
