Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 5000 pg/ml | 2.193 | 2.269 | 2.134 |
| 2.344 | |||
| 2500 pg/ml | 1.347 | 1.278 | 1.143 |
| 1.208 | |||
| 1250 pg/ml | 0.741 | 0.722 | 0.587 |
| 0.702 | |||
| 625 pg/ml | 0.434 | 0.421 | 0.286 |
| 0.408 | |||
| 312.5 pg/ml | 0.266 | 0.263 | 0.128 |
| 0.26 | |||
| 156.25 pg/ml | 0.197 | 0.191 | 0.056 |
| 0.185 | |||
| 78.125 pg/ml | 0.161 | 0.166 | 0.031 |
| 0.171 | |||
| 0 pg/ml | 0.126 | 0.135 | 0 |
| 0.144 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 180 | 6.3 | 3.5 |
| Intra-assay | 2 | 20 | 562 | 16.9 | 3 |
| Intra-assay | 3 | 20 | 2677 | 118 | 4.4 |
| Inter-assay | 1 | 20 | 180 | 24.5 | 13.6 |
| Inter-assay | 2 | 20 | 558 | 66.3 | 11.9 |
| Inter-assay | 3 | 20 | 2661 | 283 | 10.6 |
Protocols
References
Customer reviews and Q&As
