Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2500 pg/ml | 2.922 | 2.833 | 2.73 |
2.744 | |||
1250 pg/ml | 1.462 | 1.465 | 1.362 |
1.468 | |||
625 pg/ml | 0.736 | 0.77 | 0.667 |
0.804 | |||
312.5 pg/ml | 0.439 | 0.44 | 0.337 |
0.44 | |||
156.25 pg/ml | 0.251 | 0.268 | 0.165 |
0.285 | |||
78.125 pg/ml | 0.189 | 0.185 | 0.082 |
0.181 | |||
39.0625 pg/ml | 0.15 | 0.151 | 0.048 |
0.151 | |||
0 pg/ml | 0.106 | 0.103 | 0 |
0.1 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 293 | 10.8 | 3.7 |
Intra-assay | 2 | 20 | 650 | 16.7 | 2.6 |
Intra-assay | 3 | 20 | 1806 | 46.4 | 2.6 |
Inter-assay | 1 | 20 | 291 | 22.7 | 7.8 |
Inter-assay | 2 | 20 | 647 | 44.4 | 6.9 |
Inter-assay | 3 | 20 | 1808 | 133 | 7.3 |
Protocols
References
Customer reviews and Q&As