Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1800 pg/ml | 2.277 | 2.3 | 2.173 |
2.323 | |||
900 pg/ml | 1.357 | 1.295 | 1.168 |
1.233 | |||
450 pg/ml | 0.75 | 0.718 | 0.591 |
0.686 | |||
225 pg/ml | 0.419 | 0.416 | 0.289 |
0.413 | |||
112.5 pg/ml | 0.275 | 0.269 | 0.142 |
0.263 | |||
56.25 pg/ml | 0.183 | 0.187 | 0.06 |
0.191 | |||
28.125 pg/ml | 0.152 | 0.156 | 0.029 |
0.159 | |||
0 pg/ml | 0.119 | 0.127 | 0 |
0.135 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 189 | 11 | 5.8 |
Intra-assay | 2 | 20 | 651 | 26.3 | 4 |
Intra-assay | 3 | 20 | 1356 | 106 | 7.8 |
Inter-assay | 1 | 20 | 188 | 15.6 | 8.3 |
Inter-assay | 2 | 20 | 658 | 57 | 8.7 |
Inter-assay | 3 | 20 | 1351 | 133 | 9.8 |
Protocols
References
Customer reviews and Q&As