Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1500 pg/ml | 2.188 | 2.271 | 2.184 |
2.354 | |||
750 pg/ml | 1.299 | 1.295 | 1.208 |
1.292 | |||
375 pg/ml | 0.699 | 0.732 | 0.645 |
0.766 | |||
187.5 pg/ml | 0.424 | 0.418 | 0.331 |
0.412 | |||
93.75 pg/ml | 0.242 | 0.258 | 0.171 |
0.275 | |||
46.875 pg/ml | 0.174 | 0.171 | 0.084 |
0.169 | |||
23.4375 pg/ml | 0.142 | 0.136 | 0.049 |
0.13 | |||
0 pg/ml | 0.09 | 0.087 | 0 |
0.085 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 261 | 17.5 | 6.7 |
Intra-assay | 2 | 20 | 556 | 15.9 | 2.9 |
Intra-assay | 3 | 20 | 1135 | 29.4 | 2.6 |
Inter-assay | 1 | 20 | 262 | 26 | 9.9 |
Inter-assay | 2 | 20 | 560 | 41.9 | 7.5 |
Inter-assay | 3 | 20 | 1124 | 69.7 | 6.2 |
Protocols
References
Customer reviews and Q&As