Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1000 pg/ml | 2.93 | 2.917 | 2.833 |
2.903 | |||
500 pg/ml | 1.501 | 1.56 | 1.476 |
1.618 | |||
250 pg/ml | 0.817 | 0.861 | 0.777 |
0.905 | |||
125 pg/ml | 0.475 | 0.476 | 0.392 |
0.476 | |||
62.5 pg/ml | 0.303 | 0.299 | 0.215 |
0.294 | |||
31.25 pg/ml | 0.182 | 0.191 | 0.107 |
0.199 | |||
15.625 pg/ml | 0.124 | 0.129 | 0.045 |
0.133 | |||
0 pg/ml | 0.079 | 0.084 | 0 |
0.089 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 70.2 | 4 | 5.7 |
Intra-assay | 2 | 20 | 260 | 12.3 | 4.7 |
Intra-assay | 3 | 20 | 605 | 30.7 | 5.1 |
Inter-assay | 1 | 20 | 70.6 | 3.7 | 5.3 |
Inter-assay | 2 | 20 | 260 | 10.9 | 4.2 |
Inter-assay | 3 | 20 | 612 | 42.9 | 7 |
Protocols
References
Customer reviews and Q&As