Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
600 pg/ml | 2.435 | 2.539 | 2.424 |
2.643 | |||
300 pg/ml | 1.441 | 1.422 | 1.307 |
1.402 | |||
150 pg/ml | 0.76 | 0.79 | 0.675 |
0.819 | |||
75 pg/ml | 0.437 | 0.447 | 0.332 |
0.456 | |||
37.5 pg/ml | 0.301 | 0.282 | 0.167 |
0.262 | |||
18.75 pg/ml | 0.193 | 0.197 | 0.082 |
0.2 | |||
9.375 pg/ml | 0.156 | 0.149 | 0.034 |
0.141 | |||
0 pg/ml | 0.112 | 0.115 | 0 |
0.117 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 30.6 | 2.6 | 8.5 |
Intra-assay | 2 | 20 | 82.6 | 3.9 | 4.7 |
Intra-assay | 3 | 20 | 344 | 17 | 5 |
Inter-assay | 1 | 20 | 30.5 | 2.7 | 8.7 |
Inter-assay | 2 | 20 | 81.8 | 5 | 6.1 |
Inter-assay | 3 | 20 | 342 | 22.9 | 6.7 |
Protocols
References
Customer reviews and Q&As