Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
400 pg/ml | 2.562 | 2.513 | 2.42 |
2.465 | |||
200 pg/ml | 1.404 | 1.473 | 1.38 |
1.542 | |||
100 pg/ml | 0.797 | 0.832 | 0.739 |
0.867 | |||
50 pg/ml | 0.458 | 0.464 | 0.371 |
0.47 | |||
25 pg/ml | 0.244 | 0.252 | 0.159 |
0.261 | |||
12.5 pg/ml | 0.19 | 0.181 | 0.088 |
0.173 | |||
6.25 pg/ml | 0.132 | 0.137 | 0.044 |
0.142 | |||
0 pg/ml | 0.094 | 0.093 | 0 |
0.092 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 59.3 | 3.4 | 5.7 |
Intra-assay | 2 | 20 | 121 | 4.3 | 3.5 |
Intra-assay | 3 | 20 | 275 | 14.5 | 5.3 |
Inter-assay | 1 | 20 | 59.1 | 3.7 | 6.3 |
Inter-assay | 2 | 20 | 121 | 12.4 | 10.3 |
Inter-assay | 3 | 20 | 274 | 8.9 | 3.3 |
Protocols
References
Customer reviews and Q&As