Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
400 pg/ml | 1.464 | 1.525 | 1.415 |
1.586 | |||
200 pg/ml | 0.987 | 0.923 | 0.813 |
0.859 | |||
100 pg/ml | 0.511 | 0.535 | 0.425 |
0.559 | |||
50 pg/ml | 0.305 | 0.325 | 0.215 |
0.345 | |||
25 pg/ml | 0.209 | 0.197 | 0.087 |
0.185 | |||
12.5 pg/ml | 0.144 | 0.153 | 0.043 |
0.162 | |||
6.25 pg/ml | 0.139 | 0.133 | 0.023 |
0.127 | |||
0 pg/ml | 0.11 | 0.11 | 0 |
0.109 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 29.6 | 1.5 | 5.1 |
Intra-assay | 2 | 20 | 82.1 | 2.8 | 3.4 |
Intra-assay | 3 | 20 | 293 | 7.5 | 2.5 |
Inter-assay | 1 | 20 | 29.7 | 2.6 | 8.8 |
Inter-assay | 2 | 20 | 81.7 | 8.3 | 10.2 |
Inter-assay | 3 | 20 | 290 | 26.9 | 9.3 |
Protocols
References
Customer reviews and Q&As