Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
250 pg/ml | 2.549 | 2.466 | 2.335 |
2.383 | |||
125 pg/ml | 1.32 | 1.361 | 1.23 |
1.402 | |||
62.5 pg/ml | 0.823 | 0.793 | 0.662 |
0.763 | |||
31.25 pg/ml | 0.452 | 0.475 | 0.344 |
0.498 | |||
15.625 pg/ml | 0.29 | 0.307 | 0.176 |
0.324 | |||
7.8125 pg/ml | 0.202 | 0.213 | 0.082 |
0.224 | |||
3.90625 pg/ml | 0.165 | 0.175 | 0.044 |
0.185 | |||
0 pg/ml | 0.139 | 0.131 | 0 |
0.123 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 18.6 | 1.3 | 7.2 |
Intra-assay | 2 | 20 | 64.2 | 4.4 | 6.9 |
Intra-assay | 3 | 20 | 131 | 6.1 | 4.6 |
Inter-assay | 1 | 20 | 18.4 | 1.9 | 10.1 |
Inter-assay | 2 | 20 | 63.7 | 6.1 | 9.6 |
Inter-assay | 3 | 20 | 131 | 10 | 7.7 |
Protocols
References
Customer reviews and Q&As