3β-HSD7 Polyclonal Antibody

BT-AP00031                                      WB,ELISA

Description

Cat.No. BT-AP00031  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB, ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human HSD3B7. AA range:121-170

Protein Name: 3 beta-hydroxysteroid dehydrogenase type 7

Species reactivity: human, mouse, rat

Concentration: 1 mg/ml

Observed molecular weight: 41 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 1:2000

ELISA: 1:20000

Not yet tested in other applications

Synonyms: HSD3B7; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; C(27) 3-beta-HSD

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

HSD3B7 encodes an enzyme (hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7) which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in HSD3B7 are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for HSD3B7.