4.1G Polyclonal Antibody

BT-AP00032                                      WB,IHC-p,ELISA

Description

Cat.No. BT-AP00032  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p, ELISA

Source: Rabbit

Immunogen: Synthesized peptide derived from human protein. AA range: 170-250

Protein Name: Band 4.1-like protein 2

Species reactivity: human

Concentration: 1 mg/ml

Observed molecular weight: 112 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100 – 1: 300

ELISA: 1: 40000

Not yet tested in other applications

Synonyms: EPB41L2; Band 4.1-like protein 2; Generally expressed protein 4.1; 4.1G

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

The 4.1 gene family encodes a group of multifunctional cytoskeletal proteins (4.1R, 4.1G, 4.1N and 4.1B), which are predominantly expressed in the nervous system. 4.1G is a protein that stabilizes spectrin-actin interactions and is associated with hereditary elliptocytosis. Red blood cell 4.1, designated 4.1R, is a multifunctional protein that is essential for maintaining erythrocyte shape and membrane mechanical properties. Both 4.1R and 4.1G are distributed in a unique pattern in the cerebellum and are believed to modulate the membrane mechanical properties of neuronal cells by promoting fodrin/actin association. 4.1N and 4.1B, designated EPB41L1 and EPB41L3, respectively, are strongly expressed in the brain. Antibodies to 4.1N have been reported to detect mulitple forms, each enriched in postsynaptic density preparations relative to brain homogenate. Antibodies to 4.1B have been reported to detect two forms.