Cat.No. BT-AP00036 Size: 100ul/50ul/20ul Datasheet Request
Immunogen: The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:4-53
Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
Species reactivity: human, mouse, rat
Concentration: 1 mg/ml
Observed molecular weight: 18 kDa
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
WB: 1:500 – 1:2000
IHC: 1:100 – 1:300
Not yet tested in other applications
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.
EIF4EBP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.