4E-BP1 Polyclonal Antibody

BT-AP00039                                      WB,IHC-p,ELISA

Description

Cat.No. BT-AP00039  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:13-62

Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1

Species reactivity: human, mouse, rat

Concentration: 1 mg/ml

Observed molecular weight: 18 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100 – 1:300

ELISA: 1:20000

Not yet tested in other applications

Synonyms: EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

EIF4EBP1 encodes one member (eukaryotic translation initiation factor 4E binding protein 1) of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.