BUB1 Polyclonal Antibody

BT-AP01009                                      WB,ELISA

Description

Cat.No. BT-AP01009  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB, ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830

Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1

Species reactivity: human

Concentration: 1 mg/ml

Observed molecular weight: 120 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Synonyms:

BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

Recommended Dilution:

WB: 1:500 – 1:2000

ELISA: 1: 40000

Not yet tested in other applications

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

BUB1 mitotic checkpoint serine/threonine kinase(BUB1) Homo sapiens This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.