c-Abl Polyclonal Antibody

BT-AP01071                                      WB,IHC-p,IF,ELISA

Description

Cat.No. BT-AP01071  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,IF,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human ABL1. AA range:861-910

Protein Name: Tyrosine-protein kinase ABL1

Species reactivity: Human, mouse, rat

Concentration: 1 mg/ml

Observed molecular weight: 125 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100-1:300

IF: 1:200 – 1:1000

ELISA: 1:20000

Not yet tested in other applications

Synonyms: ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

ABL proto-oncogene 1, non-receptor tyrosine kinase(ABL1) Homo sapiens This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5′ end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.