C/EBP alpha Polyclonal Antibody

BT-AP01015                                      WB,IHC-p,ELISA

Description

Cat.No. BT-AP01015  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human C/EBP-alpha. AA range:192-241

Protein Name: CCAAT/enhancer-binding protein alpha

Species reactivity: human, mouse, rat, monkey

Concentration: 1 mg/ml

Observed molecular weight: 37 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Synonyms:

CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100-1: 300

ELISA: 1: 5000

Not yet tested in other applications

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

CCAAT/enhancer binding protein alpha(CEBPA) Homo sapiens This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.