C4a/b Polyclonal Antibody

BT-AP01048                                      WB,IHC-p,ELISA

Description

Cat.No. BT-AP01048  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human C4A/C4B. AA range:721-770

Protein Name: Complement C4-A/Complement C4-B

Species reactivity: Human

Concentration: 1 mg/ml

Molecular weight: 180 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100 – 1:300

ELISA: 1:5000 – 1:20000

Not yet tested in other applications

Synonyms: C4A; CO4; CPAMD2; Complement C4-A; Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4B; CO4; CPAMD3; Complement C4-B; Basic complement C4; C3 and PZP-like alph

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

complement C4A (Rodgers blood group)(C4A) Homo sapiens This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene.