CA VB Polyclonal Antibody

BT-AP01061                                      WB,IHC-p,IF,ELISA

Description

Cat.No. BT-AP01061  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,IF,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human CA5B. AA range:241-290

Protein Name: Carbonic anhydrase 5B mitochondrial

Species reactivity: Human, mouse, rat

Concentration: 1 mg/ml

Observed molecular weight: 38 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100 – 1:300

IF: 1:200- 1:1000

ELISA: 1:10000

Not yet tested in other applications

Synonyms: CA5B; Carbonic anhydrase 5B; mitochondrial; Carbonate dehydratase VB; Carbonic anhydrase VB; CA-VB

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

carbonic anhydrase 5B(CA5B) Homo sapiens Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.