CAD Polyclonal Antibody

BT-AP01094                                      IHC-p,ELISA

Description

Cat.No. BT-AP01094  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: IHC-p,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human CAD. AA range:422-471

Protein Name: CAD protein

Species reactivity: Human,mouse

Concentration: 1 mg/ml

Observed molecular weight: 250 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

IHC: 1:100 – 1: 300

ELISA: 1:20000

Not yet tested in other applications

Synonyms: CAD; CAD protein

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase(CAD) Homo sapiens The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.