CAF-1 p60 Polyclonal Antibody

BT-AP01108                                      WB,IHC-p,IF,ELISA

Description

Cat.No. BT-AP01108  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,IF,ELISA

Source: Rabbit

Immunogen: The antiserum was produced against synthesized peptide derived from human CAF1B. AA range:71-120

Protein Name: Chromatin assembly factor 1 subunit B

Species reactivity: human, mouse, rat

Concentration: 1 mg/ml

Observed molecular weight: 61 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Synonyms: CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; Chromatin assembly factor 1 subunit B; CAF-1 subunit B; Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7

Recommended Dilution:

WB: 1:500 – 1:2000

IHC: 1:100 -1:300

IF: 1:200 – 1:1000

ELISA: 1:5000 – 1:20000

Not yet tested in other applications

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

Chromatin assembly factor 1 subunit B(CHAF1B) Homo sapiens Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.