Histone H3 Monoclonal Antibody(1G1)

BT-MCA0796                                      WB,IHC-p,IF,IP

Description

Cat.No. BT-MCA0796  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,IF,IP

Source: Mouse

Immunogen: Recombinant Protein of Histone H3.

Protein Name: Histone H3.1/Histone H3.2/Histone H3.3.

Species reactivity: Human,Mouse,Rat,Yeast

Concentration: 1 mg/ml

ConcentraObserved molecular weight: 15 kDa

Formulation

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycero.

Purification:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Recommended Dilution:

WB: 1:2000 – 5000

IF:1:100 – 500

IP: 1:200

IHC: 1:50 – 300

Not yet tested in other applications

Synonyms: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.