Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(5D8)

BT-MCA0792                                      WB

Description

Cat.No. BT-MCA0792  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Polyclonal

Applications: WB

Source: Mouse

Immunogen: Synthetic Peptide of Histone H3 (Di Methyl Lys79).

Protein Name: Histone H3.1/Histone H3.2/Histone H3.3.

Species reactivity: Human,Mouse,Rat

Concentration: 1 mg/ml

ConcentraObserved molecular weight: 15 kDa

Formulation

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Recommended Dilution:

WB: 1:1000 – 3000

Not yet tested in other applications

Synonyms: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H3A is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from HIST1H3A lack polyA tails; instead, they contain a palindromic termination element. HIST1H3A is found in the large histone gene cluster on chromosome 6p22-p21.3.