HP-1 Gamma mouse Monoclonal Antibody(4F4)

BT-MCA0823                                      WB,IHC-p

Description

Cat.No. BT-MCA0823  Size: 100ul/50ul/20ul    Datasheet Request  

Product Information

Clonallity: Monoclonal

Applications: WB,IHC-p

Source: Mouse

Immunogen: Recombinant Protein of HP-1 gamma.

Protein Name: Chromobox protein homolog 3 (HECH) (Heterochromatin protein 1 homolog gamma) (HP1 gamma) (Modifier 2 protein).

Species reactivity: Human,Mouse,Rat

Concentration: 1 mg/ml

ConcentraObserved molecular weight: 24 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Recommended Dilution:

WB: 1:500 – 2000

IHC-p: 1:50 – 300

Not yet tested in other applications

Synonyms: Chromobox protein homolog 3 (HECH) (Heterochromatin protein 1 homolog gamma) (HP1 gamma) (Modifier 2 protein)

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Background:

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by CBX3 (chromobox 3) binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5′ UTR, have been found for CBX3.