Human Muscarinic Acetylcholine Receptor M4 ELISA kit

E3026Hu                                               48 wells/96 wells

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Description

This sandwich kit is for the accurate quantitative detection of human Muscarinic Acetylcholine Receptor M4 (also known as M-ACHR M4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Cat.No E3026Hu

Standard Curve Range: 0.05ng/ml – 15ng/ml

Sensitivity: 0.024ng/ml   Datasheet Download

Storage: The kit could be stored for 6 months under the temperature of 2-8°C and could be stored for longer time under the temperature below -20°C. The ELISA plate is packed in vacuum package and the strip is dismountable. If it’s not used up once after the dismounting, put it in the provided seal bag and put in the drying agent and store it under the temperature of 2-8°C. It could be used again within one month. Prevent the repeated frost-thawing for the reagent.

Kit Contents

  • Standard Solution
  • Pre-coated ELISAPlate
  • Standard Diluent
  • Streptavidin-HRP
  • Stop Solution
  • Substrate Solution A
  • Substrate Solution B
  • Wash Buffer Concentrate (25x)
  • Biotin-Conjugate Antibody
  • User Instruction
  • Plate Sealer
  • Zipper bag

References

“Novel signaling pathways mediating reciprocal control of keratinocyte migration and wound epithelialization through M3 and M4 muscarinic receptors.”
Chernyavsky A.I., Arredondo J., Wess J., Karlsson E., Grando S.A.
J. Cell Biol. 166:261-272(2004)

“Muscarinic M4 receptor recycling requires a motif in the third intracellular loop.”
Hashimoto Y., Morisawa K., Saito H., Jojima E., Yoshida N., Haga T.
J. Pharmacol. Exp. Ther. 325:947-953(2008)