FAQs

No Signal

  • Improper standard dilution
  • Inccorect storage of reagents
  • Incomplete washing of the wells
  • Capture antibody did not bind to theplate

  Solution

  • Reconstitute standard according to the
  • Store the reagents in the ELISA kit according to the printed instruction before using them
  • Make sure wells are washed adequately by filling the wells with wash buffer and all residual antibody solutions crossed well before washing.
  • Replace a new ELISA plate

High Background

  • Improper washing
  • Substrate was contaminated
  • Non-specific binding of antibody
  • Plate are not be sealing incompletely
  • Incorrect incubation temperature
  • Substrate exposed to light prior to use
  • Contaminated wash buffer

  Solution

  • Increasing duration of soaking steps
  • Substrate should be clean and avoid  crossed contamination by using the sealer
  • Replace another puritified antibody or blocking buffer
  • Make sure to follow the instruction strictly
  • Incubate at room temperature
  • Keep substrate in a dark place
  • Use a clean buffers and sterile filter.

Weak Signal

  • Improper washing
  • Incorrect incubation temperature
  • Antibody are not enough
  • Reagent are contaminated
  • Pipette are not clean

Solution

  • Increasing duration of soaking steps
  • Incubate at room temperature
  • Increase the concentration of the antibody
  • Use new one
  • Pipette should be clean

Poor Standard Curve

  • Improper standard dilution
  • Inccorect storage of reagents
  • Incomplete washing of the wells
  • Capture antibody did not bind to theplate

Solution

  • Reconstitute standard according tothe
  • Store the reagents in the ELISA kit according to theprinted instruction before using them
  • Make sure wells are washed adequatelyby filling the wells with wash buffer and all residual antibody solutions crossed well before washing
  • Replace a new ELISA plate