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14-3-3 theta/tau(Phospho Ser232) Polyclonal Antibody
Description
| Product type | Primary Antibody |
| Code | BT-AP00027 |
| Host | Rabbit |
| Isotype | IgG |
| Size | 100ul, 50ul, 20ul |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232. AA range:196-245 |
| Mol wt | 27764 |
| Species reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Recommended application | WB, IHC-p, IF, ICC, ELISA |
| Concentration | 1 mg/ml |
| Full name | 14-3-3 protein theta |
| Synonyms | YWHAQ; YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
This product is for research use only, not for use in human, therapeutic or diagnostic procedure.
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Recommended Dilution
WB: 1: 500 - 1: 2000
IHC-p: 1: 100 - 1: 300
IF: 1: 200 - 1: 1000
ICC: 1: 200 - 1: 1000
ELISA: 1: 5000
Not yet tested in other applications.
IHC-p: 1: 100 - 1: 300
IF: 1: 200 - 1: 1000
ICC: 1: 200 - 1: 1000
ELISA: 1: 5000
Not yet tested in other applications.
Images
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Immunofluorescence analysis of Hela cell. 14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody(Green) was diluted at 1:200(4°C overnight). (Red) was diluted at 1:200(4°C overnight). |
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Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western blot analysis of 293T MOUSE-BRAIN using p-14-3-3 θ/τ (S232) antibody. Antibody was diluted at 1:500 |
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Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The lane on the right is blocked with the phospho peptide. |
Storage
-20°C for 1 year