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4E-BP1 Polyclonal Antibody
Description
Product type |
Primary Antibody |
Code |
BT-AP00036 |
Host |
Rabbit |
Isotype |
IgG |
Size |
20ul, 50ul, 100ul |
Immunogen |
The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:4-53 |
Mol wt |
12449 |
Species reactivity |
Human, Mouse, Rat |
Clonality |
Polyclonal |
Recommended application |
WB, IHC-p, ELISA |
Concentration |
1 mg/ml |
Full name |
4E-BP1 Antibody |
Synonyms |
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I |
This product is for research use only, not for use in human, therapeutic or diagnostic procedure.
Background
EIF4EBP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Recommended Dilution
WB: 1: 500 - 1: 2000
IHC: 1: 100 - 1: 300
ELISA: 1: 5000
Not yet tested in other applications.
Images
|
Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF 200ng/ml 30', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide. |
|
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide. |
Storage
-20°C for one year