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Home > Product > ELISA Kit > Mouse Bile Salt-activated Lipase, CEL ELISA Kit

Mouse Bile Salt-activated Lipase, CEL ELISA Kit

Mouse Bile Salt-activated Lipase, CEL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.29ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.29ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Bile salt-activated lipase

Code

E1884Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.29ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs) (PubMed:27509211). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (PubMed:27509211). Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins (By similarity).

UniProt accession

MASS(Da)

65,813

GeneID

12613

Synonyms

1810036E18Rik;BAL;Bile salt-activated lipase;Bile salt-stimulated lipase;BSSL;Carboxyl ester lipase;CEL;Cholesterol esterase;Pancreatic lysophospholipase;Sterol esterase

Gene names

Cel

Research area

Others

Target protein

CEL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1884Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.879 1.943 1.893
2.007
600ng/L 0.998 1.052 1.003
1.107
300ng/L 0.43 0.46 0.411
0.49
150ng/L 0.191 0.223 0.173
0.255
75ng/L 0.13 0.109 0.06
0.088
0ng/L 0.045 0.049 0
0.053

Precision

We measured random samples of E1884Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 734.48 15.76 2.15
Intra-Assay 2 18 541.07 19.43 3.59
Intra-Assay 3 18 286.41 11.97 4.18

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1884Mo

    $320.00

  • 96T

    E1884Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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