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Home > Product > ELISA Kit > Mouse Cystatin-A, CSTA ELISA Kit

Mouse Cystatin-A, CSTA ELISA Kit

Mouse Cystatin-A, CSTA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.17ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.17ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Cystatin-A

Code

E1989Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.17ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cystatin-A is a protein that in humans is encoded by the CSTA gene. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

UniProt accession

MASS(Da)

10,929

GeneID

209294

Synonyms

Cs;Csta;Cystatin-A;Cystatin-A1;Stefin-A

Gene names

Csta

Research area

Cell Biology

Target protein

CSTA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1989Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.264 2.26 2.207
2.257
2400ng/L 1.902 1.859 1.805
1.816
1200ng/L 1.289 1.186 1.132
1.082
600ng/L 0.681 0.663 0.609
0.644
300ng/L 0.414 0.392 0.338
0.369
0ng/L 0.057 0.054 0
0.05

Precision

We measured random samples of E1989Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1518.6 63.24 4.16
Intra-Assay 2 18 542.37 25.46 4.7
Intra-Assay 3 18 1034.92 64.1 6.19

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1989Mo

    $320.00

  • 96T

    E1989Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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