Full product name
Cytochrome P450 1B1
Code
E3398Hu
Assay type
Sandwich
Size
48T,96T
Sensitivity
0.235ng/ml
Detection range
0.5-150ng/ml
Sample type
Serum, plasma, cell culture supernates
Species
Human
Storage
2-8ºC
Assay time
1h 30m
Background
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 20972997, PubMed: 11555828, PubMed: 12865317, PubMed: 10681376, PubMed: 15258110). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 20972997, PubMed: 11555828, PubMed: 12865317, PubMed: 10681376, PubMed: 15258110). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position (PubMed: 11555828, PubMed: 12865317). Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (PubMed: 10426814). May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376, PubMed: 15258110). Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system (PubMed: 20972997). Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2 (PubMed: 21068195). Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (PubMed: 10426814). Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (By similarity).
UniProt accession
MASS（Da）
60,846
GeneID
1545
Synonyms
CYP1B1;CYPIB1;Cytochrome P450 1B1;Hydroperoxy icosatetraenoate dehydratase
Gene names
CYP1B1
Research area
Others
Target protein
CYP1B1
