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Home > Product > ELISA Kit > Mouse Lymphocyte Antigen 96, LY96 ELISA Kit

Mouse Lymphocyte Antigen 96, LY96 ELISA Kit

Mouse Lymphocyte Antigen 96, LY96 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Lymphocyte antigen 96

Code

E2222Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Lymphocyte antigen 96, also known as "Myeloid Differentiation factor 2 (MD-2)," is a protein that in humans is encoded by the LY96 gene. The protein encoded by this gene is involved in binding lipopolysaccharide with Toll-Like Receptor (TLR4). The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccharide (LPS), thus providing a link between the receptor and LPS signaling.[7] That is, the primary interface between TLR4 and MD-2 is formed before binding LPS and the dimerization interface is induced by binding LPS.

UniProt accession

MASS(Da)

18,394

GeneID

17087

Synonyms

ESO;ESOP-1;LY96;Ly-96;Lymphocyte antigen 96;MD2;MD-2;Protein MD-2

Gene names

Ly96

Research area

Immunology

Target protein

LY96

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2222Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.169 2.275 2.239
2.381
6ng/mL 1.494 1.568 1.533
1.642
3ng/mL 1.138 1.132 1.097
1.126
1.5ng/mL 0.664 0.686 0.651
0.707
0.75ng/mL 0.454 0.465 0.43
0.477
0ng/mL 0.042 0.035 0
0.028

Precision

We measured random samples of E2222Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.36 0.12 9.17
Intra-Assay 2 18 1.62 0.13 7.94
Intra-Assay 3 18 4.04 0.19 4.69

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2222Mo

    $320.00

  • 96T

    E2222Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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