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Home > Product > ELISA Kit > Rat Thioredoxin-interacting Protein, TXNIP ELISA Kit

Rat Thioredoxin-interacting Protein, TXNIP ELISA Kit

Rat Thioredoxin-interacting Protein, TXNIP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.95ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 9.95ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Thioredoxin-interacting protein

Code

E2277Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.95ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth (By similarity).

UniProt accession

MASS(Da)

44,018

GeneID

117514

Synonyms

Thioredoxin-interacting protein;TXNIP;Vdup1;Vitamin D3 up-regulated protein 1

Gene names

Txnip

Research area

Others

Target protein

TXNIP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Thioredoxin-interacting Protein, TXNIP ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.065 2.11 2.069
2.155
1600ng/L 1.244 1.236 1.195
1.229
800ng/L 0.856 0.755 0.713
0.653
400ng/L 0.521 0.493 0.452
0.465
200ng/L 0.212 0.194 0.152
0.175
0ng/L 0.035 0.041 0
0.048

Precision

We measured random samples of E2277Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 475.02 32.74 6.89
Intra-Assay 2 18 2629.2 78.02 2.97
Intra-Assay 3 18 857.74 48.48 5.65

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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