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Home > Product > ELISA Kit > Mouse Pancreatic Alpha Amylase, AMY2A ELISA Kit

Mouse Pancreatic Alpha Amylase, AMY2A ELISA Kit

Mouse Pancreatic Alpha Amylase, AMY2A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.23mU/mL

Detection range: 5-1000mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 2.23mU/mL

Detection range: 5-1000mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Pancreatic alpha amylase

Code

E2326Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.23mU/mL

Detection range

5-1000mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Pancreatic alpha-amylase is an enzyme that in humans is encoded by the AMY2A gene. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

UniProt accession

MASS(Da)

57,318

GeneID

100043684;100043686;100043688;109959

Synonyms

1,4-alpha-D-glucan glucanohydrolase;Amy;Amy-;Amy2;Amy-2;AMY2A;mAmy2-;mAmy2-2;PA;Pancreatic alpha-amylase

Gene names

Amy2

Research area

Others

Target protein

AMY2A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Pancreatic Alpha Amylase, AMY2A ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640mU/mL 1.703 1.839 1.777
1.975
320mU/mL 1.08 1.082 1.02
1.084
160mU/mL 0.438 0.475 0.413
0.513
80mU/mL 0.263 0.266 0.204
0.269
40mU/mL 0.079 0.102 0.04
0.125
0mU/mL 0.063 0.062 0
0.062

Precision

We measured random samples of E2326Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 432.75 11.59 2.68
Intra-Assay 2 18 147.17 5.51 3.75
Intra-Assay 3 18 356.43 15.67 4.4

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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