Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse Thioredoxin-interacting Protein, TXNIP ELISA Kit

Mouse Thioredoxin-interacting Protein, TXNIP ELISA Kit

Mouse Thioredoxin-interacting Protein, TXNIP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.98ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.98ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Thioredoxin-interacting protein

Code

E2512Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.98ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.

UniProt accession

MASS(Da)

44,363

GeneID

56338

Synonyms

1200008J08Rik;AA682105;Hyp;Hyplip1;mVDU;Tbp-2;THI;THIF;Thioredoxin-interacting protein;TXNIP;VDU;Vitamin D3 up-regulated protein 1

Gene names

Txnip

Research area

Signal Transduction

Target protein

TXNIP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2512Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 1.916 1.871 1.836
1.827
1000ng/L 1.082 1.035 0.999
0.988
500ng/L 0.545 0.483 0.448
0.421
250ng/L 0.251 0.269 0.234
0.287
125ng/L 0.138 0.101 0.065
0.063
0ng/L 0.038 0.036 0
0.033

Precision

We measured random samples of E2512Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1130.14 62.28 5.51
Intra-Assay 2 18 1229.48 34.33 2.79
Intra-Assay 3 18 368.73 15.95 4.33

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2512Mo

    $320.00

  • 96T

    E2512Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up