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Home > Product > ELISA Kit > Human Calmodulin-regulated Spectrin-associated Protein 2, CAMSAP1L1 ELISA Kit

Human Calmodulin-regulated Spectrin-associated Protein 2, CAMSAP1L1 ELISA Kit

Human Calmodulin-regulated Spectrin-associated Protein 2, CAMSAP1L1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Calmodulin-Regulated Spectrin-Associated Protein 2

Code

E3649Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed: 23169647, PubMed: 24486153, PubMed: 24706919). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed: 24486153, PubMed: 24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed: 24486153, PubMed: 24706919). In addition, it also reduces the velocity of microtubule polymerization (PubMed: 24486153, PubMed: 24706919). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed: 27666745). Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed: 27666745). Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (PubMed: 24908486). Through the microtubule cytoskeleton, regulates the autophagosome transport (PubMed: 28726242).

UniProt accession

MASS(Da)

168,089

GeneID

23271

Synonyms

Calmodulin-regulated spectrin-associated protein 1-like protein 1;Calmodulin-regulated spectrin-associated protein 2;CAMSAP 2;CAMSAP2

Gene names

CAMSAP2

Research area

Others

Target protein

CAMSAP2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3649Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.087 2.077 2.024
2.068
4ng/ml 1.234 1.298 1.246
1.363
2ng/ml 0.699 0.748 0.696
0.798
1ng/ml 0.448 0.456 0.404
0.465
0.5ng/ml 0.216 0.207 0.154
0.197
0ng/ml 0.063 0.053 0
0.042

Precision

We measured random samples of E3649Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.5 0.22 4
Intra-Assay 2 18 0.9 0.06 6.3
Intra-Assay 3 18 3.6 0.23 6.3

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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