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Home > Product > ELISA Kit > Human Adipolin, AD ELISA Kit

Human Adipolin, AD ELISA Kit

Human Adipolin, AD ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Adipolin

Code

E3692Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue.

UniProt accession

MASS(Da)

32,416

GeneID

388581

Synonyms

Adipolin;Adipolin cleaved form;Adipolin fC1QTNF12;Adipolin fCTRP12;Adipolin full-length form;Adipolin gC1QTNF12;Adipolin gCTRP12;Adipose-derived insulin-sensitizing factor;C 1QTNF12;C1q and TNF related protein 12;C1QTNF12;Complement C1q tumor necrosis factor-related protein 12

Gene names

C1QTNF12

Research area

Cell Biology

Target protein

C1QTNF12

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.222 2.239 2.182
2.257
6ng/ml 1.34 1.362 1.304
1.384
3ng/ml 0.894 0.862 0.804
0.83
1.5ng/ml 0.411 0.437 0.379
0.462
0.75ng/ml 0.257 0.273 0.215
0.288
0ng/ml 0.065 0.058 0
0.05

Precision

We measured random samples of E3692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.7 0.1 6.1
Intra-Assay 2 18 5.1 0.33 6.5
Intra-Assay 3 18 5.6 0.23 4.2

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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