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Home > Product > ELISA Kit > Human Cystathionine Gamma-lyase, CTH ELISA Kit

Human Cystathionine Gamma-lyase, CTH ELISA Kit

Human Cystathionine Gamma-lyase, CTH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.24ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.24ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cystathionine gamma-lyase

Code

E5143Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.24ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.

UniProt accession

MASS(Da)

44,508

GeneID

1491

Synonyms

CSE;CTH;Cystathionine gamma-lyase;Cysteine-protein sulfhydrase;Gamma-cystathionase

Gene names

CTH

Research area

Signal Transduction

Target protein

CTH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5143Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.029 2.115 2.053
2.2
1200ng/L 1.878 1.802 1.741
1.727
600ng/L 1.092 1.099 1.038
1.106
300ng/L 0.685 0.719 0.658
0.752
150ng/L 0.419 0.407 0.346
0.396
0ng/L 0.059 0.061 0
0.063

Precision

We measured random samples of E5143Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 482.69 27.33 5.66
Intra-Assay 2 18 1333.33 100.51 7.54
Intra-Assay 3 18 297.91 19.1 6.41

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5143Hu

    $320.00

  • 96T

    E5143Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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