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Home > Product > ELISA Kit > Human Ecto-nox Disulfide-Thiol Exchanger 2, ENOX2 ELISA Kit

Human Ecto-nox Disulfide-Thiol Exchanger 2, ENOX2 ELISA Kit

Human Ecto-nox Disulfide-Thiol Exchanger 2, ENOX2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.59ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.59ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ecto-NOX disulfide-thiol exchanger 2

Code

E5221Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.59ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.

UniProt accession

MASS(Da)

70,082

GeneID

10495

Synonyms

APK1;APK1 antigen;COVA1;Cytosolic ovarian carcinoma antigen 1;Ecto-NOX disulfide-thiol exchanger 2;ENOX2;Includes: Hydroquinone [NADH] oxidase;Protein disulfide-thiol oxidoreductase;tNOX;Tumor-associated hydroquinone oxidase

Gene names

ENOX2

Research area

Cancer

Target protein

ENOX2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5221Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.042 1.979 1.919
1.917
1200ng/L 1.111 1.063 1.002
1.015
600ng/L 0.516 0.529 0.469
0.542
300ng/L 0.23 0.265 0.205
0.301
150ng/L 0.156 0.148 0.088
0.14
0ng/L 0.064 0.06 0
0.057

Precision

We measured random samples of E5221Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1913.38 52.71 2.75
Intra-Assay 2 18 964.57 35.54 3.68
Intra-Assay 3 18 810.39 40.74 5.03

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5221Hu

    $320.00

  • 96T

    E5221Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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