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Home > Product > ELISA Kit > Human Plasma Kallikrein, KLKB1 ELISA Kit

Human Plasma Kallikrein, KLKB1 ELISA Kit

Human Plasma Kallikrein, KLKB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.11mU/mL

Detection range: 5-1000mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.11mU/mL

Detection range: 5-1000mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Plasma kallikrein

Code

E5880Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.11mU/mL

Detection range

5-1000mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

UniProt accession

MASS(Da)

71,370

GeneID

3818

Synonyms

Fletcher factor;Kininogenin;KLK3;KLKB1;PKK;PKKD;Plasma kallikrein;Plasma kallikrein heavy chain;Plasma kallikrein light chain];Plasma prekallikrein;PPK

Gene names

KLKB1

Research area

Cardiovascular; Signal Transduction; Cancer; Cell Biology

Target protein

KLKB1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5880Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640mU/mL 1.864 1.959 1.908
2.054
320mU/mL 0.955 1.009 0.959
1.063
160mU/mL 0.405 0.48 0.43
0.555
80mU/mL 0.281 0.298 0.247
0.315
40mU/mL 0.098 0.107 0.057
0.117
0mU/mL 0.059 0.05 0
0.042

Precision

We measured random samples of E5880Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 433.71 10.15 2.34
Intra-Assay 2 18 362.2 17.89 4.94
Intra-Assay 3 18 144.6 6.85 4.73

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5880Hu

    $320.00

  • 96T

    E5880Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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