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Home > Product > ELISA Kit > Human Protein Delta Homolog 1, DLK1 ELISA Kit

Human Protein Delta Homolog 1, DLK1 ELISA Kit

Human Protein Delta Homolog 1, DLK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.47ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.47ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein delta homolog 1

Code

E5959Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.47ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein delta homolog 1 is a protein that in humans is encoded by the DLK1 gene. It is expressed as a transmembrane protein, but a soluble form cleaved off by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes. It is a member of the EGF-like family of homeotic proteins. Part of the Dlk1-DIO3 imprinting control region, this gene is one involved in the epigenetic process that causes a subset of genes to be regulated based on their parental origin. Such imprinted genes are required for the formation of the placenta as well as the development of cellular lineages such as those derived from the mesoderm and ectoderm.

UniProt accession

MASS(Da)

41,300

GeneID

8788

Synonyms

DLK;DLK1;DLK-1;FA1;FA1];Fetal antigen 1;pG2;PREF1;Pref-1;Protein delta homolog 1;ZOG

Gene names

DLK1

Research area

Signal Transduction; Neuroscience; Stem Cells

Target protein

DLK1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5959Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.231 2.233 2.194
2.234
1600ng/L 1.528 1.516 1.478
1.505
800ng/L 0.81 0.848 0.809
0.886
400ng/L 0.551 0.482 0.444
0.414
200ng/L 0.272 0.284 0.245
0.295
0ng/L 0.036 0.039 0
0.041

Precision

We measured random samples of E5959Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1086.07 49.57 4.56
Intra-Assay 2 18 1367.86 47.25 3.45
Intra-Assay 3 18 450.58 22.11 4.91

Protocols

References (1)

DLK1 and Nesfatin-1 levels and the relationship with metabolic parameters in polycystic ovary syndrome: Prospective, controlled study

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  • Size

    Code

    Price

  • 48T

    E5959Hu

    $320.00

  • 96T

    E5959Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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