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Home > Product > ELISA Kit > Canine Interleukin 10, IL-10 ELISA Kit

Canine Interleukin 10, IL-10 ELISA Kit

Canine Interleukin 10, IL-10 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.02pg/ml

Detection range: 2-700pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 1.02pg/ml

Detection range: 2-700pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Interleukin 10

Code

E0006Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.02pg/ml

Detection range

2-700pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

UniProt accession

MASS(Da)

20,906

GeneID

403628

Synonyms

CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names

IL10

Research area

Immunology; Cancer

Target protein

IL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0006Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400pg/ml 2.164 2.176 2.127
2.188
200pg/ml 1.777 1.749 1.7
1.721
100pg/ml 1.132 1.114 1.065
1.096
50pg/ml 0.704 0.712 0.663
0.721
25pg/ml 0.35 0.352 0.303
0.353
0pg/ml 0.067 0.049 0
0.031

Precision

We measured random samples of E0006Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 72.2 3.66 5.1
Intra-Assay 2 18 178.6 9.09 5.1
Intra-Assay 3 18 43.7 3.11 7.1

Protocols

References (1)

Comparison of some cytokines, acute phase proteins and citrulline levels in healthy and canine distemper infected dogs

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  • Size

    Code

    Price

  • 96T

    E0006Ca

    $475.00

  • 48T

    E0006Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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