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Home > Product > ELISA Kit > Human Calpain-5, CAPN5 ELISA Kit

Human Calpain-5, CAPN5 ELISA Kit

Human Calpain-5, CAPN5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Calpain-5

Code

E6587Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.11ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

UniProt accession

MASS(Da)

73,169

GeneID

726

Synonyms

ADNIV;Calpain htra-3;Calpain-5;CAPN5;HTRA3;nCL-3;New calpain 3;VRNI

Gene names

CAPN5

Research area

Signal Transduction; Cell Biology

Target protein

CAPN5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Calpain-5, CAPN5 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.304 2.358 2.288
2.413
1200ng/L 1.349 1.384 1.314
1.418
600ng/L 0.815 0.775 0.705
0.735
300ng/L 0.505 0.529 0.458
0.552
150ng/L 0.312 0.296 0.226
0.28
0ng/L 0.07 0.07 0
0.07

Precision

We measured random samples of E6587Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1024.52 66.3 6.47
Intra-Assay 2 18 1539.01 51.95 3.38
Intra-Assay 3 18 266.17 17.63 6.62

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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