Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Calpain-5, CAPN5 ELISA Kit

Human Calpain-5, CAPN5 ELISA Kit

Human Calpain-5, CAPN5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Calpain-5

Code

E6587Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.11ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

UniProt accession

MASS(Da)

73,169

GeneID

726

Synonyms

ADNIV;Calpain htra-3;Calpain-5;CAPN5;HTRA3;nCL-3;New calpain 3;VRNI

Gene names

CAPN5

Research area

Signal Transduction; Cell Biology

Target protein

CAPN5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6587Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.304 2.358 2.288
2.413
1200ng/L 1.349 1.384 1.314
1.418
600ng/L 0.815 0.775 0.705
0.735
300ng/L 0.505 0.529 0.458
0.552
150ng/L 0.312 0.296 0.226
0.28
0ng/L 0.07 0.07 0
0.07

Precision

We measured random samples of E6587Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1024.52 66.3 6.47
Intra-Assay 2 18 1539.01 51.95 3.38
Intra-Assay 3 18 266.17 17.63 6.62

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E6587Hu

    $320.00

  • 96T

    E6587Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up