Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Chymotrypsin-like Elastase Family Member 3B, CELA3B ELISA Kit

Human Chymotrypsin-like Elastase Family Member 3B, CELA3B ELISA Kit

Human Chymotrypsin-like Elastase Family Member 3B, CELA3B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Chymotrypsin-like elastase family member 3B

Code

E6849Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.053ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Chymotrypsin-like elastase family member 3B also known as elastase-3B, protease E, or fecal elastase is an enzyme that in humans is encoded by the CELA3B gene. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

UniProt accession

MASS(Da)

29,263

GeneID

23436

Synonyms

CBPP;CELA3B;Chymotrypsin-like elastase family member 3B;ELA3B;Elastase IIIB;Elastase-3B;Protease E

Gene names

CELA3B

Research area

Signal Transduction; Cell Biology

Target protein

CELA3B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6849Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 1.778 1.847 1.795
1.916
12ng/ml 1.118 1.051 0.999
0.984
6ng/ml 0.42 0.495 0.442
0.569
3ng/ml 0.271 0.26 0.208
0.25
1.5ng/ml 0.107 0.112 0.06
0.118
0ng/ml 0.05 0.052 0
0.054

Precision

We measured random samples of E6849Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 12.25 0.46 3.76
Intra-Assay 2 18 16.12 0.39 2.42
Intra-Assay 3 18 7.86 0.3 3.78

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E6849Hu

    $320.00

  • 96T

    E6849Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up