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Home > Product > ELISA Kit > Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit

Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit

Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.42ng/L

Detection range: 5-1800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.42ng/L

Detection range: 5-1800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Bcl2 Antagonist of Cell Death

Code

E4102hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.42ng/L

Detection range

5-1800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

UniProt accession

MASS(Da)

18,392

GeneID

572

Synonyms

BAD;Bcl2 antagonist of cell death;Bcl2-associated agonist of cell death;Bcl-2-binding component 6;Bcl2-L-8;Bcl-2-like protein 8;Bcl-xL/Bcl-2-associated death promoter

Gene names

BAD

Research area

Cancer

Target protein

BAD

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4102hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000ng/L 1.995 1.875 1.826
1.755
500ng/L 0.951 0.918 0.869
0.885
250ng/L 0.548 0.49 0.441
0.432
125ng/L 0.24 0.245 0.196
0.25
62.5ng/L 0.067 0.105 0.057
0.144
0ng/L 0.052 0.049 0
0.046

Precision

We measured random samples of E4102hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 602.8 25.41 4.2
Intra-Assay 2 18 795.6 22.8 2.9
Intra-Assay 3 18 248.9 11.95 4.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4102hu

    $320.00

  • 96T

    E4102hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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