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Home > Product > ELISA Kit > Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit

Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit

Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.56ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.56ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cyclin Dependent Kinase 9

Code

E4386hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.56ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein kinase involved in the regulation of transcription (PubMed: 10574912, PubMed: 10757782, PubMed: 11145967, PubMed: 11575923, PubMed: 11809800, PubMed: 11884399, PubMed: 14701750, PubMed: 16109376, PubMed: 16109377, PubMed: 20930849, PubMed: 28426094). Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP (PubMed: 10574912, PubMed: 10757782, PubMed: 11145967, PubMed: 11575923, PubMed: 11809800, PubMed: 11884399, PubMed: 14701750, PubMed: 16109376, PubMed: 16109377, PubMed: 20930849, PubMed: 28426094). This complex is inactive when in the 7SK snRNP complex form (PubMed: 10574912, PubMed: 10757782, PubMed: 11145967, PubMed: 11575923, PubMed: 11809800, PubMed: 11884399, PubMed: 14701750, PubMed: 16109376, PubMed: 16109377, PubMed: 20930849, PubMed: 28426094). Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELF (PubMed: 9857195, PubMed: 10912001, PubMed: 11112772, PubMed: 12037670, PubMed: 20081228, PubMed: 20980437, PubMed: 21127351). Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling) (PubMed: 17956865, PubMed: 18362169). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis (PubMed: 10393184, PubMed: 11112772). P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export (PubMed: 15564463, PubMed: 19575011, PubMed: 19844166). Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing (PubMed: 15564463, PubMed: 19575011, PubMed: 19844166). The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro (PubMed: 21127351). Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage (PubMed: 20493174). In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6 (PubMed: 20493174). Promotes cardiac myocyte enlargement (PubMed: 20081228). RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription (PubMed: 21127351). AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect (PubMed: 9857195, PubMed: 10912001, PubMed: 11112772). The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (PubMed: 12037670).

UniProt accession

MASS(Da)

42,778

GeneID

1025

Synonyms

C-2K;CDK 9;CDK9;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Cyclin-dependent kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit

Gene names

CDK9

Research area

Cell Biology; Microbiology; Epigenetics and Nuclear Signaling; Immunology

Target protein

CDK9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4386hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.925 1.973 1.923
2.022
800ng/L 0.954 0.964 0.913
0.973
400ng/L 0.47 0.482 0.432
0.494
200ng/L 0.248 0.23 0.18
0.212
100ng/L 0.142 0.154 0.103
0.165
0ng/L 0.064 0.05 0
0.036

Precision

We measured random samples of E4386hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 631.3 27.71 4.4
Intra-Assay 2 18 1291.1 32.47 2.5
Intra-Assay 3 18 487.3 23.38 4.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E4386hu

    $320.00

  • 96T

    E4386hu

    $458.00

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Lead time: Within two weeks

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